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      Burkert Contromatic Corp. US Headquarters
      11425 Mt. Holly-Huntersville Road
      Huntersville, NC 28078
      Phone +1 800 325 1405
      Fax +1 949 223 3198
      E-Mail znexrgvat-hfn@ohexreg.pbz
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      Sales Office - West Coast Burkert Contromatic Corp.
      2572 White Road
      Irvine, CA 92614
      Phone +1 800 325 1405
      Fax +1 949 223 3198
      E-Mail znexrgvat-hfn@ohexreg.pbz
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Liquid chromatography

"Chromatography" is an umbrella term covering physical methods in which substances are separated via distribution between a stationary and mobile phase.

HPLC (High-Performance Liquid Chromatography) is a separating process in which a liquid sample is transported through a stationary phase under high pressure. Depending on the nature of the interaction between the phases and the sample, we distinguish between adsorption chromatography, partition chromatography, ion-exchange chromatography, sizeexclusion chromatography and affinity chromatography. The processes of adsorption chromatography and partition chromatography are the chief processes used in HPLC. HPLC uses partition particles with grain sizes of a few µm; it thus achieves high numbers of plates or trays, but at the same time, requires a high counter-pressure to be overcome during transport of the mobile phase through the thin separation column (of a few millimeters diameter). All parts must be interconnected without any dead volume wherever possible and must be pressure-stable (up to 300 bar).

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